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TB-4 vs TB-500 Thymosin Beta-4 (TB-4) is a naturally occurring peptide in the human body, composed of 43 amino acids

For double labelling, Alexa Fluor 568 streptavidin conjugate (Invitrogen, 1:250) was used for visualising vascular staining (IB4), donkey anti-mouse (Alexa Fluor 488 conjugate, Invitrogen, 1:250) and donkey anti-rabbit Alexa Fluor 488 conjugate (Invitrogen) diluted in 1% normal horse serum in PBS was used for visualising immunostatining of IGF-1 receptors

It is a tripeptide with a gamma peptide linkage between the carboxyl group of the glutamate side chain and the amine group of cysteine, and the carboxyl group of cysteine is attached by normal peptide linkage to a glycine

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Depending on results and providers discretion the injections may be repeated up to 3-4 times per year for sustained pain relief