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Description

A series of three experiments were designed to evaluate Ovagens efficacy in promoting follicular growth, its influence on preovulatory follicle characteristics, and potential carryover effects on subsequent reproductive cycles [1]

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Cells were lysed at 4C in icecold radioimmunoprecipitation assay lysis buffer (Millipore, Billerica, MA, USA) or 3[(3cholamidopropyl)dimethylammonio]1propane sulphonate lysis buffer (Cell Signaling Technology) as indicated in figure legends