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For coimmunoprecipitation assays, WT human IR and L973F-IR cDNA were cloned into 3_Flag-CMV-14 mammalian expression vector (MilliporeSigma)

The recommended approach is to start by testing thyroid peroxidase (TPO) and thy roid globulin antibodies

Kazanskaya, O

Sometimes patients will report a mild asthma flare during testing, which to their surprise is rapidly relieved by the administration of the individuals neutralizing dose for that trigger