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Methods Computational analysis of human thyroid cancers in the cancer genome atlas database (TCGA) We queried the TCGA database of PTCs utilizing the Firehouse web browser from the Broad Institute ( and utilized TIMER2.0 ( a publicly available computational software, for analyses of tumor clinical and genomic features 35

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The final pellets were air-dried and resuspended in the appropriate sample buffers and then subjected to SDS-PAGE and two-dimensional (2D) gel electrophoresis, followed by Flamingo fluorescence gel staining analysis (Bio-Rad Laboratories, Hercules, CA) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) analysis at the University of Pittsburgh Health Sciences Mass Spectrometry Core

While sulphites dont trigger an allergic (IgE-mediated) reaction, they can cause non-allergic hypersensitivity responses leading to asthma-like symptoms, digestive upset, or headaches (PMID: 1576344)