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10.1007/s13760-020-01349-8 Acta Neurol

Cell Culture The human colon cancer cell lines HCT116 and SW620, purchased from American Type Culture Collection (Manassas, VA, USA), were cultured in RPMI medium and DMEM/F12 (HyClone, Logan, UT, USA) respectively, supplemented with 10% foetal bovine serum (HyClone) and 1% penicillin and streptomycin

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Barrier dysfunction due to unavoidable injuries or allergic responses enhances the release of IL-1, leading to the increased formation of cytokine proteins [183, 184]