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IP samples were subsequently centrifuged at 200 x g for 2 min at 4C, supernatant removed, and beads washed five times with 1 mL low stringency lysis buffer

Since changes in intracellular Ca2+ elicited by hyper or hypocalcemia depend on the expression and activity of the CaSR, any decrease explains, at least in part, an impaired intracellular calcium response to [Ca2+e] and hence a reduced inhibitory effect of the cation on PTH secretion

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Sacpic staining results also showed that the proportion of primary and secondary active hair follicles has no significant change