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10.1016/j.cell.2019.03.032 7 Ali-RahmaniF.SchengrundC

Colorimetric Detection reagent Dilution table 1/2 Plate one Plate two Plates four Plates Colorimetric Detection Concentrate 140 L 260 L 500 L 1 mL Assay Buffer 1.26 mL 2.34 mL 4.5 mL 9 mL total Colorimetric reagent Volume 1.4 mL 2.6 mL 5 mL 10 mL reaction Mixture Prepare the Reaction Mixture by diluting one part each NADPH and Glutathione Reductase Concentrates 1:10 into eight parts Assay Buffer