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By inhibiting this enzyme, 5-Amino-1MQ preserves nicotinamide for NAD+ synthesis, increasing intracellular NAD+ levels through a mechanism entirely distinct from NAD+ precursor supplementation (NMN, NR)

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By blocking NNMT, 5-amino-1MQ stimulates an increase in nicotinamide adenine dinucleotide (NAD+), a cofactor that is central to cellular metabolism, thereby increasing metabolic rate and activating a gene called sirtuin-1 (SIRT1)

This happens through several interconnected pathways