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Donelli (Ed.), Advances in microbiology, infectious diseases and public health (Vol

For crystallization of ShHTL4, 0.40 L of protein solution (18.0 mg mL 1 ) was mixed with 0.40 L of reservoir solution containing 90 mM Tris (pH 9.0), 180 mM MgCl 2 , 27% (w/v) PEG4000, and 1 mM GSH and incubated at 20 C