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Thiamin deficiency is also observed as a complication of the refeeding syndrome: the introduction of carbohydrates in severely starved individuals leads to an increased demand for thiamin in glycolysis and the citric acid cycle that precipitates thiamin deficiency (27)

Step 8: Label the vial immediately

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The Dwarfs of Sindh: severe growth hormone (GH) deficiency caused by a mutation in the GH-releasing hormone receptor gene

For the generation of triple mutants, the kanamycin cassette was removed from the gshA, gsiC or gshA, gsiB double deletion mutant using the plasmid 709-FLPe as indicated by the supplier (Gene Bridges, Heidelberg, Germany)