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Immunofluorescence Cells grown on coverslips were fixed with cold methanol at 20 C and then incubated with the following primary antibodies: anti-LC3A/B rabbit monoclonal (1:100) (Cell Signaling Technology, Inc., New England Biolabs., UK), anti-Phospho-S6 Ribosomal Protein rabbit monoclonal (1:100) (Cell Signaling Technology), anti p53 mouse monoclonal (1:100) (Dako Cytomation, Glostrup, Denmark) and anti-Ki67 rabbit polyclonal (1:300) (Abcam, Cambridge Science Park, Cambridge, UK)

Association of trace element abnormalities and adverse outcomes in patients with acute heart failure

Biocatalystartificial metalloenzyme cascade based on alcohol dehydrogenase

Often called the "master antioxidant," it is found in almost every cell in the human body

Figure 2 Among neural interactions, microglia-neuron crosstalk has been the most intensively studied, particularly during brain development