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Monday + Thursday) Total per week: 45 mg Aggressive loading (severe / acute injuries): 5 mg per injection , twice weekly = 10 mg/week Conservative loading (prevention / budget): 2.5 mg per injection , twice weekly = 5 mg/week Duration: 46 weeks Total peptide for standard loading: approximately 4050 mg (standard) or 80 mg (aggressive) TB-500 maintenance phase dosage Standard maintenance dose: 22.5 mg once weekly Reduced maintenance: 2 mg every 2 weeks (for long-term joint support) Duration: 48 weeks after loading

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The CDO activity state is regulated by Cys concentration via a substrate-turnover-dependent formation of a thioether cross-link between the sulfur of residue Cys93 and the aromatic side chain of residue Tyr157