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On the next day, after the membrane was washed with TBST for five times, HRP-conjugated secondary antibody (Thermo Scientific) was applied for 1.5 h at room temperature

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The reason for this is that the initial wave of drug-induced hepatocellular destruction is followed by a robust innate immune response, in which invading inflammatory cells release toxic oxidants and cause a second wave of destruction

Mercapturic acids profiling has been recently proposed by Gonalves-Diaset et al

One strategy involves post-conditioning after stroke with bouts of mild hypoxia