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For pituisphere transcriptome sequencing we generated and amplified 62 cDNA from tubes containing pituispheres using REPLI-G WTA single cell kit according to manufacturers instructions

Setchell KD, Brown NM, Summer S, et al

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As consequences of polymorphism of MTHFR , reduction of MTHFR enzymatic activity would cause impaired methylation as well as deficiency of folate