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Graefe's Arch Clin Exp Ophthalmol

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Indeed, the CuAAC can be performed site-selectively with complete conversion 34 and has been used in many significant applications, such as the generation of PEGylated proteins 87 , the generation of dual PTM glycoprotein mimics due to its orthogonality to existing cysteine chemistry 34,35 , cellular proteomic analysis (BONCAT) 80 , a quantitative method for primary cell proteomics (QuaNCAT) 88 , and the construction of highly-valent protein nanoparticles 89

Most patients begin noticing improvements in skin texture and tone within three to six weeks of consistent use