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After overnight incubation, the membranes were washed in TBS-Tween and then incubated with the following secondary antibodies for 1 h at room temperature: Goat Anti-Rabbit IgG(H + L)HRP(H + L;1:5000, sparkjed, Cat#EF0002)

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Du Toit, 2023

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The neurons of the cortical cerebral, cerebellar region, hippocampus, and hypothalamus were counted in 10 different high-powered fields (HPF, 400x) and 3 to 5 serial sections of each sample were used to do the count as described in The field size was 0.24 m 2