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Description

[PubMed: 11700945] 198

Immunoprecipitation and Western blot HEK293T cells were collected and lysed in lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, protease inhibitors and benzonase) and incubated on ice for 30 min

Electrophiles capable of undergoing substitution reactions have a leaving group , a species that can accept and stabilize the pair of electrons that make up the bond being broken