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Calcium, diacylglycerol, cAMP and various phosphate derivatives of myoinositol (phosphatidylinositol and others) are essential secondary messengers involved in the regulation of intracellular signaling cascades, in response to the activation of the TSHR, and are necessary for the production H 2 O 2 and TG synthesis ( In patients with latent HT with a high titer of TPOAb and the presence of mild iodine deficiency, the content of Ca, Mg and Zn in blood serum decreases (138)

[112] Furthermore, this assay requires overnight incubation and may give false results if any inactive vitamin B 12 analogues are present in the foods