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After washing 3 20min with 1ml 0,2% gelatin-TBS secondary antibody incubation occurred as described for the primary antibody and the coverslips were incubated for one hour at room temperature

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The generally accepted guideline is 28 days from first puncture

The natural 3D channels of sugarcane stems were preserved through freeze-drying, followed by carbonization and KOH activation to explode into 2D arc-shaped carbon nanosheets (2DPC90, Fig

When residues L43, W53, and F54 were substituted with alanine, the inhibitory ability was significantly reduced, indicating that these hydrophobic residues were essential for FOXO4 FHD binding (Table 1)