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A (Life Technologies), 1x N2 (Life Technologies), 1x PenStrep, 1x GlutaMAX (Life Technologies), 0,075% sodium bicarbonate (Life Technologies), 0.5 x MEM-NEAA (Life Technologies), 200 nM ascorbic acid (Sigma)) supplemented with SB431542 (Tocris, 10M), XAV939 (VWR, 2M) and LDN-193189 (Sigma, 100nM)