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2021 Oct 1;27(5):1321-1343

This method is based on the color reaction between allyl thiourea and potassium ferricyanide solution in dilute acetic acid which measures the optical density of the color complex at 600 nm and it works in the range of 2.5 to 7.5 picogram of AITC per milliliter of the color solution

Notably, the proportion of CD3 + and CD8 + dual-positive cytotoxic T cells was significantly higher in the combination therapy group (5.64 1.72%)14.8-fold that in the anti-PD-1 group (0.38 0.11%) and 3.97-fold that in the monotherapy group (1.42 0.59%) (Fig

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