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doi: 10.1186/s12967-022-03320-y 173

A Spectra Systems FL2000 fluorometer (Spectra Physics, Mountain View, California, USA) was used for detection, with excitation and emission at 394 and 480 nm, respectively

Then the calibration curve was plotted using absorbance obtained against different concentrations

A case-control study found significantly decreased risk of age-related cataracts (33% to 51%) in men and women in the highest quintile of dietary riboflavin intake (median of 1.6 to 2.2 mg/day) compared to those in the lowest quintile (median of 0.08 mg/day in both men and women) (33)

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