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Genes that were significantly differentially expressed between primary tumours and PM were identified using a cut off of P primary gene set was strongly correlated with expression of the 143 CMS4 classifier genes in the CRC-PM cohort analysed in this study ( r = 0.813), and even better in the large composite CMS cohort consisting of 3232 primary CRC tumours ( r = 0.900) (Fig

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Based on the 4-point Likert scale, raw item scores were converted into dichotomous variables for LCA: scores 1 were assigned to the low-response group (coded as 0)

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