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Intracellular ROS production was detected using 2,7-dichlorofluorescein diacetate (DCFH-DA) as a chemical probe

Thal possesses various pharmacological properties, including anti-inflammatory, antifibrotic and anti-angiogenic (Chong et al., 2006

Additionally, the upregulated genes relevent to iron homeostasis also include gene ecoding ferritin light chain (FTL) protein, the gene encoding microtubule-associated proteins 1 A/1B light chain 3B (MAP1LC3B) and the gene encoding nuclear receptor coactivator (NCOA4)

The protein precipitate was removed via centrifugation and supernatants chromatographed using a Shimadzu LC system equipped with an Atlantis C 18 column (186001291

The gold standard for diagnosis is an oral food challenge (OFC), ideally a double-blind placebo-controlled food challenge (DBPCFC)