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OPA-derivatized amino acids were detected using 1260 Infinity II Fluorescence Detector with excitation of 340 nm and emission of 450 nm

Requena JR, Levine RL, Stadtman ER

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Results Oxidized or reduced glutathione reduces Grx1-roGFP2 in cells lacking glutathione biosynthesis, but not in glutathione reductase-deficient cells The genetically encoded redox probe roGFP2 contains two cysteines that, when oxidized, form a disulfide, changing the excitation properties of the probe

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