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Mol Cell Biol 29: 27042715.

This depends on your desired concentration and dose

RNA extraction was carried out using Trizol (Sigma) and gcs-1 , gst-7 , gst-4 , dhs-8 , sdz-8 and act-1 mRNA levels were determined using Superscript III Platinum SYBR Green One-Step qRT-PCR kit (Invitrogen, Paisley, UK) and Corbett Life Science Rotor-Gene 6000 system

Similar to the previously discussed study, the research team of Khavinson et al

Whole blood was collected via carotid artery laceration under isoflurane anesthesia, and plasma was collected from blood by centrifugation (2000 g, 20 min, 4 C) for cytokine analysis