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HFD could also promote the overgrowth of pathogenic bacteria in the intestine and induce the maturation of proinflammatory immune cells, leading to uncontrolled inflammation and mucosal damage indirectly, which were unable to inhibit the overgrowth of pathogenic bacteria, further aggravating the pathophysiological state (Statovci et al., 2017)

*Correspondence: Mauro Magnani, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

The vector pQTG has been derived from pQE30N, a modified pQE-30 vector (Qiagen), that has an additional Not I-restriction site in the multiple cloning site (obtained from Eberhard Scherzinger)