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en.wikipedia.org/wiki/Hypom

Pharmacol Rep 72:16451656

doi: 10.1080/19490976.2024.2414805 92 LiHYangYYangYZhaiCYaoJLiaoWet al

In some structures of activated-IdeR, the third metal (cobalt)-binding site located on the surface of Domain 3 was also observed, and this metal is coordinated by His219, His223, and four water molecules

the invention includes embodiments of fusion proteins comprising an intein N-fragment and a purification tag, wherein the purification tag is located at the intein split junction, which is the C-terminus of the intein N-fragment, wherein the intein N-fragment carries a mutation that abolishes N-terminal cleavage activity