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The cells were homogenized and the plates incubated for 30 min at 4 C

The accumulation of ROS has been demonstrated to trigger the phosphorylation and activation of the key kinase p38 MAPK, suggesting that ROS is a potential activator of the MAPK signaling pathway [75]

Characterization of the endotheliopathy, innate-immune activation and hemostatic imbalance underlying CAR-T cell toxicities: laboratory tools for an early and differential diagnosis