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As NAD+ levels drop with age, our cells become more vulnerable to damage

In brief, isolated cells were lyzed in 500 L Qiazol following sorting, 100 L chloroform were added and samples spun at 2500 g for 15 min at +4 C in a MaXtract column (Qiagen, Venlo, Netherlands)

Berk BC, Pereira A, Vizcarra VS, Prschel C, Hsu CG Frontiers in immunology

10.1016/j.bcp.2015.06.013 122 LinM.LuS.-s.WangA.-x.QiX.-y.ZhaoD.WangZ.-h.et al (2011a)

Western blot analysis of SOD1 and CA3 showed that these proteins were present in APAP overdose patients and were not observed in the pooled control sample