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Finally, the secondary antibody is washed 5 x 5 minutes in TBS-T before being imaged using chemiluminescence Clarity Western ECL or Clarity Max Western ECL on an LAS4000 (GE)

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Although GABA synthesis may initially support neuron survival, the increase in MAO-B expression for GABA synthesis may result in elevated production of hydrogen peroxide, which may worsen the condition (Chun et al., 2020)