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The overnight-grown subcultures were transferred into 96-well plates over 24 h, and their growth curves were measured for cell density (OD 600 ) using a Tecan Spark plate reader (Tecan Group Ltd., Mannedorf, Switzerland)

Garcia L, Garcia F, Llorens F, Unzeta M, Itarte E, Gomez N (2002) PP1/PP2A phosphatases inhibitors okadaic acid and calyculin A block ERK5 activation by growth factors and oxidative stress