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Undetected and untreated, B12 deficiency can lead to a range of clinical problems, including reversible megaloblastic anemia and demyelinating neurologic disease

Following electroporation, cells were incubated for 48 h in recovery medium (RPMI-1640 supplemented with 100 non-essential amino acids (Gibco) and 1 mM sodium pyruvate (Gibco)), followed by sorting for GFP (BD FACS Aria III)

Signal Transduct Target Ther 8:267 Wu D, Re D, Nagai M et al (2006) The inflammatory NADPH oxidase enzyme modulates motor neuron degeneration in amyotrophic lateral sclerosis mice