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RNA, DNA, and protein quantification Cultured and thawed synovial MSCs and HUVECs (3 10 5 cells) were suspended in 250 L of 95% FBS and 5% DMSO (Fujifilm Wako Pure Chemical Corporation, Tokyo, Japan)

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Baseline metabolic testing and hormone panels set a baseline, which should be regularly monitored, including glucose metrics, reproductive hormones, and ultrasound as appropriate

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Freeman, 2017)