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The following acquisition settings were used: AGC (MS) = 3 10 6 , AGC (MS 2 ) = 1 10 5 , maximum injection time (MS) = 200 ms, maximum injection time (MS 2 ) = 50 ms, mass range = 2601,900 Da, resolution MS = 70,000 (FWHM at m / z 200), resolution MS 2 = 35,000 (FWHM at m / z 200), MS 2 spectra were acquired in the top-10 ions in each cycle, isolation window = 1.0 m / z , dynamic exclusion = 12 s and normalized collision energy = 2530

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