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Clean the injection site with an alcohol prep pad, pinch a fold of skin, insert the needle at a 45-90 degree angle (depending on body fat levels), inject slowly over 3-5 seconds, and dispose of used syringes immediately in a sharps container

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These results suggest that NNT loss-of-function leads to reductive stress through increasing NADH accumulation and decreasing NADPH production, and that NNT can integrate with the PDH complex to form a redox circuit regulating H 2 O 2 production