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An equal volume of buffer composed of 32% sucrose, 30% glycerol, 10 mmol/L MgCl 2 , 10 mmol/L KH 2 PO 4 (pH 7.4), 1 mmol/L PMSF, and protease inhibitor cocktail was added to the mixture and shaken for another 15 min

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Rev Anal Chem 38(4):20190019

In the present study, the improved immune quality of colostrum and the higher concentration of plasma TIG in calves injected with micronutrients could explain the lower inflammatory response and enhanced immune homeostasis in these groups compared to the control

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