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doi: 10.1016/j.bbrc.2025.152541 124 BaoZHuaLYeYWangDLiCXieQet al

after an initial 5-min warm up, the running speed was gradually increased to 25 m/min, which was maintained for 30 min

Coronal sections (250 M thick) from a hypothalamic block were cut with a Leica VT1000S vibratome and then incubated in oxygenated aCSF at room temperature for at least 1 h before recording