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The mixture was incubated on ice for 5 minutes before being centrifuged at 21,000 g for 5 minutes at 4 C

Nat Rev Microbiol (2020) 19:7794

In their experimental models, GHRH alone was estimated to increase growth hormone burst by roughly 20-fold over baseline, whereas GHRP-2 alone was associated with an even larger 47-fold rise