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Fluorescent substrates (Dendra or sfGFP) containing the N-end rule degron and His14-SUMO tag were purified by Ni-NTA resin, followed by the SUMO tag cleavage, SUMO tag removal with Ni-NTA resin, and gel filtration, similarly to the purification of Ufd1 proteins

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This approach helps maintain treatment consistency and avoids missed doses

Oxidative stress may then damage mitochondrial membranes and enzymes, further lowering ATP production and increasing the need for glutathione, SOD, catalase and other antioxidant defenses

Vitamin E Fact Sheet for Health Professionals