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In some embodiments, the antioxidant has a logP of about -0.27

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Its size and susceptibility to proteolysis have historically limited oral absorption

Evaluation of In Vitro Methionine -Lyase Cytotoxicity on HT29 Cell Line by MTS Assay and Trypan Blue Exclusion Assay HT29 cells were treated with MGL at IC 50 (0.03 0.001 mg/ml, Results Section) and grown in DMEM supplemented with 5% FBS, 1% L-glutamine, and 1% penicillin/streptomycin, at 37C, 5% CO 2 , for 24, 48, and 72 h

The online software, VENNY v2.1 ( 16 was used to intersect the DEGs with all ferroptosis-related genes, redrawing the Venn Diagram with improved clarity and accuracy