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Testis tissues were excised immediately after euthanasia, rinsed with cold phosphate-buffered saline (PBS), blotted dry, and stored at 80 C

MDA, a reactive aldehyde produced during the peroxidation of polyunsaturated fatty acids, serves as a widely recognized marker of cellular oxidative stress and reflects the extent of structural compromise to membrane lipids ( 2 O 2 levels indicate an accumulation of ROS capable of damaging proteins, nucleic acids, and mitochondrial membranes, thereby disrupting cellular homeostasis and impairing tissue function ( 2 O 2 concentrations, demonstrating its potent ability to inhibit lipid peroxidation and reinforce antioxidant defenses

It has now become the most studied and widely applied strain of bakers yeast S

Endogenous glucocorticoids and bone

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