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Ann N Y Acad Sci 1033:7991

DNA and RNA Extractions, RT-PCR, and qRT-PCR For total RNA extractions, adult whiteflies (30 whiteflies/ replicate) and nymphs (50 nymphs/replicate) were collected at each time point from tomato plants and RNA was isolated using TRIzol reagent (Sigma-Aldrich) then was treated with DN ase I according to the manufactures recommendations (Thermo Scientific)

Based on mass, HDL can also be separated from other lipoproteins by size-exclusion chromatography (fast protein liquid chromatography, FPLC), and HDLs molecular weight ranges from 175,000 - 360,000 Da (762)