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It also leads to an increase in anti-inflammatory mediators such as IL-4 (116, 117)

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Zonulin as a diagnostic tool With the appreciation that zonulin is associated to a series of immune-mediated diseases, a quantitative sandwich enzyme linked immunosorbent assay (ELISA) has been developed to use its serum levels as a biomarker of intestinal barrier integrity in several inflammatory diseases (3)

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