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For mitochondria, the intensity image was first smoothed with a mean filter (radius = 2.0 pixels) and then processed with a separate pre-trained Weka classifier (200 trees, 2 features per split)

Montagner, A., et al

( B ) Western blot analysis of CD81 pulldown in Jurkat cells show that Dynabeads Protein G beads have the highest yield with a short ten-minute protein incubation