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125 l of 2.5 M calcium chloride was added to the plasmid mixture, and the final volume was made up to 1125 l using 1 mM Tris, 0.1 mM EDTA, pH 8

While Dihexa-specific desensitization kinetics haven't been thoroughly characterized in long-term studies, applying known c-Met receptor regulation patterns suggests daily dosing beyond 46 weeks without rest periods would likely produce diminishing returns

Pal et al., 2022)