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Hepatology 30:613 Venneman NG, Besselink MG, Keulemans YC, Vanberge-Henegouwen GP, Boermeester MA, Broeders IA, Go PM, van Erpecum KJ (2006) Ursodeoxycholic acid exerts no beneficial effect in patients with symptomatic gallstones awaiting cholecystectomy

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In the absence of DTT, neither MB-NO nor HMO exhibited a characteristic absorption peak at 660 nm, indicating that the disulfide bond within the MB-NO prodrug remained intact, thereby preventing its decomposition into methylene blue molecules

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