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Once reconstituted, solutions should be immediately aliquoted into single-use volumes and stored at -20C, protected from reducing agents, which can cleave the critical disulfide bond

1) results in increased release of IL-1 and TNF-, which enhances SERT expression and activity in astrocytes and, therefore, increases reuptake and degradation of 5-HT to the metabolite 5-hydroxyindoleacetic acid (5-HIAA), thereby reducing extracellular levels of 5-HT [271, 285,286,287,288]

References: Forman HJ, Zhang H, Rinna A

Indeed, reduced levels of free iron have been shown to markedly promote the growth of bladder cancer cells (87)

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