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Determination of optimum temperature and pH The optimum temperature and pH for GPx activity of rhGPx3 mutant was determined by performing enzymatic assays at different temperatures (15 to 55C) at pH 7.4

The analysis from the last decade showed that upregulated expression of Klotho is emerging as a promising therapeutic strategy for chronic kidney disease (CKD), acute kidney injury (AKI), and for cardiac hypertrophy, fibrosis and dysfunction 17,18,19

However, the expression of LAT1 on the cell membrane gradually decreased as the incubation time increased to 3 h and then significantly increased when the incubation time increased to 5 h

Ichikawa, Y

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