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In most instances, the co-existing conditions are treated simultaneously but separately, which can cause adverse effects in some patients with co-existing IBD-hepatobiliary pathology [28, 29]

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Major metabolites found were trimethylamine N-oxide, primarily in urine (8% to 49% of the administered dose) and [3H]--butyrobetaine, primarily in feces (0.44% to 45% of the administered dose)

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Each collected layer was mixed with 1 mL of PBS solution, fragmentised using a tissue homogeniser (Precellys 24) (Bertin Instrument, France) for 1 min, and centrifuged (Centrifuge 5427R) (Eppendorf Company, Germany) at 13000 rpm for 5 min